LOCUS       40924_49037        3315 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Broad host range vector vector pCVD005, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B, 
            Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Broad-host-range vector system for synthetic biology and 
            biotechnology in cyanobacteria
  JOURNAL   Nucleic Acids Res. (2014) In press
  PUBMED    25074377
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Taton A, Unglaub F, Wright N, Zeng WY, Paz Yepes J, Brahamsha B, 
            Palenik B, Peterson T, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2014) Division of Biological Sciences, University 
            of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA
REFERENCE   3  (bases 1 to 3315)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3315)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. (2014) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-JUN-2014) Division of Biological Sciences, University of 
            California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3315
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             343..354
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
     misc_feature    370..375
                     /label=EcoRV
                     /note="EcoRV"
     misc_feature    complement(373..393)
                     /note="C2G; GC-adaptor for seamless assembly, flanking 
                     antibiotic resistance markers, functional devices or custom
                     sequences; synthetic"
     misc_feature    394..399
                     /label=AgeI
                     /note="AgeI"
     promoter        406..434
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     regulatory      447..471
                     /note="luxA 5-UTR; ribosomal binding site"
                     /regulatory_class="promoter"
     CDS             472..1329
                     /label=AmpR
                     /note="beta-lactamase"
     regulatory      1341..1367
                     /note="trpA T; trpA terminator from Lorist6 INSD accession 
                     X98450, one nt shorter than reference; Lorist6"
                     /regulatory_class="terminator"
     misc_feature    1377..1382
                     /label=NheI
                     /note="NheI"
     misc_feature    complement(1383..1403)
                     /note="GC; GC-adaptor for seamless assembly, flanking
                     antibiotic resistance markers or Escherichia coli origins 
                     to be assembled with a cyanobacterial replicon; synthetic"
     misc_feature    1401..1406
                     /label=EcoRV
                     /note="EcoRV"
     rep_origin      complement(1631..2219)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2396..3187)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(3188..3292)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
      361 aggccgcatg atatccggcg gcggcggcgg gataccggtt tcgaattgac aattaatcat
      421 cggctcgtat aatggtaccc ggatcctaat accaacaaat aaggaaatgt tatgagcatc
      481 cagcactttc gcgtggccct gatccccttt tttgccgctt tttgcctgcc cgtgtttgcc
      541 caccccgaaa ccctggtgaa agtgaaagat gccgaagatc agctgggtgc tcgcgtgggc
      601 tacatcgaac tggatctgaa cagcggcaaa atcctggaaa gttttcgccc tgaagaacgc
      661 tttcccatga tgagcacctt taaagtgctg ctgtgcggtg ccgtgctgag tcgcgtggat
      721 gctggccaag aacagctggg tcgccgcatc cactacagcc agaacgatct ggtggaatac
      781 agtcccgtga ccgaaaaaca cctgaccgat ggcatgaccg tgcgcgaact gtgcagcgct
      841 gccatcacca tgagcgataa caccgctgcc aacctgctgc tgaccaccat cggtggccct
      901 aaagaactga ccgcctttct gcacaacatg ggcgatcacg tgacccgctt ggatcgctgg
      961 gaacccgaac tgaacgaagc catccccaac gatgaacgcg ataccaccat gcctgccgct
     1021 atggccacca ccctgcgcaa actgctgacg ggtgaactgc tgaccctggc cagtcgccag
     1081 cagctgatcg attggatgga agccgataaa gtggctggcc ctctgctgcg cagcgctctg
     1141 cctgctggct ggtttatcgc cgataaaagt ggcgctggcg aacgcggtag tcgcggtatc
     1201 attgccgctc tgggccccga tggcaaaccc agccgcatcg tggtgatcta caccaccggc
     1261 agccaggcca ccatggatga acgcaaccgc cagatcgccg aaatcggtgc cagcctgatc
     1321 aaacactggt aaggccggcc agcccgccta atgagcgggc ttttttttgg cgcgccgcta
     1381 gcatccgcgc gcgcgcgcgc gatatcctgg gcctcatggg ccttcctttc actgcccgct
     1441 ttccagtcgg gaaacctgtc gtgccagctg cattaacatg gtcatagctg tttccttgcg
     1501 tattgggcgc tctccgcttc ctcgctcact gactcgctgc gctcggtcgt tcgggtaaag
     1561 cctggggtgc ctaatgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     1621 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     1681 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     1741 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     1801 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     1861 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     1921 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     1981 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     2041 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     2101 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     2161 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     2221 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     2281 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     2341 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt tattagaaaa
     2401 attcatccag cagacgataa aacgcaatac gctggctatc cggtgccgca atgccataca
     2461 gcaccagaaa acgatccgcc cattcgccgc ccagttcttc cgcaatatca cgggtggcca
     2521 gcgcaatatc ctgataacga tccgccacgc ccagacggcc gcaatcaata aagccgctaa
     2581 aacggccatt ttccaccata atgttcggca ggcacgcatc accatgggtc accaccagat
     2641 cttcgccatc cggcatgctc gctttcagac gcgcaaacag ctctgccggt gccaggccct
     2701 gatgttcttc atccagatca tcctgatcca ccaggcccgc ttccatacgg gtacgcgcac
     2761 gttcaatacg atgtttcgcc tgatgatcaa acggacaggt cgccgggtcc agggtatgca
     2821 gacgacgcat ggcatccgcc ataatgctca ctttttctgc cggcgccaga tggctagaca
     2881 gcagatcctg acccggcact tcgcccagca gcagccaatc acggcccgct tcggtcacca
     2941 catccagcac cgccgcacac ggaacaccgg tggtggccag ccagctcaga cgcgccgctt
     3001 catcctgcag ctcgttcagc gcaccgctca gatcggtttt cacaaacagc accggacgac
     3061 cctgcgcgct cagacgaaac accgccgcat cagagcagcc aatggtctgc tgcgcccaat
     3121 catagccaaa cagacgttcc acccacgctg ccgggctacc cgcatgcagg ccatcctgtt
     3181 caatcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     3241 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     3301 cccgaaaagt gccac
//
